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pLC4-14 [RG100[MetJ]]
pLC4-14 [RG100[MetJ]]
規(guī)格:
貨期:
編號:B236644
品牌:Mingzhoubio

標準菌株
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DNA
RNA

規(guī)格:
凍干粉
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產(chǎn)品名稱 pLC4-14 [RG100[MetJ]]
商品貨號 B236644
Designations pLC4-14 [RG100[MetJ]]
Species Escherichia coli (Migula) Castellani and Chalmers
Depositors JR Uren
Applications
This strain overproduces beta-cystathionase 100-fold over wild type levels.
Vector
Construct size (kb): 0.0
Insert
DNA: genomic
Gene product: cystathionase beta [metC]
Media ATCC Medium 1065 (see below) plus streptomycin (100.0 mcg/ml) plus thymidine (50.0 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid
Comments
This strain overproduces beta-cystathionase 100-fold over wild type levels.
This clone was isolated from the Clarke-Carbon Escherichia coli library, constructed of hybrid Col E1 plasmid - Escherichia coli DNA using poly(dA-dT) connectors.
Classification Enterobacteriaceae, Escherichia
References

Su CH, Greene RC. Regulation of methionine biosynthesis in Escherichia coli: Mapping of the metJ locus and properties of a metJ+/metJ- diploid. Proc. Natl. Acad. Sci. USA 68: 367-371, 1971. PubMed: 5277087

Dwivedi CM, et al. Cloning, purification, and characterization of beta-cystathionase from Escherichia coli. Biochemistry 21: 3064-3069, 1982. PubMed: 7049234

Clarke L, Carbon J. A colony bank containing synthetic Col E1 hybrid plasmids representative of the entire E. coli genome. Cell 9: 91-99, 1976. PubMed: 788919

Jack R Uren, personal communication

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