Application |
The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.
The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.
Component |
Type Strain |
Volume |
Biosafety Level |
Composition |
Product Format |
Storage |
Storage Conditions |
Lysis Buffer |
梅經(jīng)理 |
17280875617 |
1438578920 |
胡經(jīng)理 |
13345964880 |
2438244627 |
周經(jīng)理 |
17757487661 |
1296385441 |
于經(jīng)理 |
18067160830 |
2088210172 |
沈經(jīng)理 |
19548299266 |
2662369050 |
李經(jīng)理 |
13626845108 |
972239479 |
金溪县|
东阿县|
邵阳市|
阿合奇县|
毕节市|
南城县|
西充县|
成都市|
安远县|
阳泉市|
延安市|
南丹县|
青浦区|
尖扎县|
临西县|
巴里|
库车县|
新巴尔虎右旗|
上林县|
贺兰县|
青岛市|
特克斯县|
会同县|
正安县|
长丰县|
定远县|
榆林市|
河北省|
阜宁县|
疏附县|
平和县|
汕头市|
尉氏县|
华宁县|
加查县|
孝义市|
马关县|
页游|
岳普湖县|
赤城县|
乐安县|
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